STUDY OF CYTOCOMPATIBILITY OF DIFFERENT SUPPLEMENTS IN GEL COMPOSITIONS USED FOR THE TREATMENT OF PERIODONTAL DISEASES IN ORTHODONTIC PATIENTS VIA EXPLORATION OF MECHANISMS OF THEIR CYTOPATHIC AND CYTOPROTECTIVE EFFECTS IN CULTURED HUMAN AND MOUSE CELLS

Introduction. A search continues for effective means which may reduce the overload of harmful factors, eliminate inflammatory process, and stress on periodontal tissues during active period orthodontic treatment. We developed patented gel composition (GC) Benzidaflaziverdine prepared based Proteflazid® (flavonoids) benzydamine hydrochloride (BH) T-Sept® local treatment in form a dressing patients.
 The aim this study was to evaluate cytocompatibility different combinations components flavonoid complex (Benzidaflaziverdine) used diseases patients. For this, mechanisms their cytopathic cytoprotective effects will be explored using cultured human mouse cells.
 Methods. studied effect supplements GC viability pseudonormal keratinocytes HaCaT line fibroblasts BALB-3T3 line, macrophages J774.2 line. Various methods cell survival assessment were used: MTT-assay, staining cells with fluorescent dyes Hoechst 33342 Propidium iodide (PI), as well test genotoxic (DNA comet assay). antioxidant properties variants evaluated DPPH (1,1-diphenyl-2-picrylhydrazyl), Merck (Dam-stadt, Germany), DCFDA-H2 (2’,7’-dichlorodihydrofluorescein diacetate).
 Results. demonstrated that Sample containing base BH solution (Tantum Verde®) possessed weak prooxidant properties. While contained base, powdered (T-Sept®) (T-Sept® Proteflazid®) pronounced properties.
 Conclusions. Tests DCFDA confirm hypothesis regarding application bandage due activity complex, reaches maximum level at 2nd hour exposure. This can recommended use clinical periodontology medical support patients before phase